In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra


  • Vanja Vuksanović University of Novi Sad, Faculty of agriculture, 21000 Novi Sad
  • Branislav Kovačević University of Novi Sad, Institute of Lowland Forestry and Environment, 21000 Novi Sad
  • Marina Katanić University of Novi Sad, Institute of Lowland Forestry and Environment, 21000 Novi Sad
  • Saša Orlović University of Novi Sad, Institute of Lowland Forestry and Environment, 21000 Novi Sad
  • Dragana Miladinović Institute of Field and Vegetable Crops, Novi Sad


European black poplar, phytoextraction, low pH, tissue culture, microwave sterilization,


Phytoextraction is an efficient and cheap way to extract copper from soils in riparian zones. In this work five genotypes of the endangered tree species Populus nigra L. were tested for their copper tolerance and accumulation in vitro when cultivated on media with three Cu concentrations: 10-3, 10-4 and 10-7 M (buffered with citric acid/Na-citrate buffer, pH 3 before sterilization). After five-weeks cultivation of rooted shoots, the highest increases in morphological and biomass parameters were observed at 10-7 M Cu2+. As the medium with 10-3 M Cu2+ exhibited a toxic effect, the effect of 10-4 M Cu2+ and pH 3 was used for further genotype evaluation. According to the measured morphological and parameters of photosynthetic pigment contents, the best performance was achieved by the genotype Populus nigra cl. DN3. The highest copper accumulation on the same medium was achieved by genotype Populus nigra cl. BN5. The obtained data point to the considerable potential of the applied method in the evaluation of Populus nigra genotypes for use in projects of copper phytoextraction.

Received: February 10, 2017; Revised: April 6, 2017; Accepted: April 26, 2017; Published online: May 15, 2017

How to cite this article: Vuksanović V, Kovačević B, Katanić M, Orlović S, Miladinović D. In vitro evaluation of copper tolerance and accumulation in Populus nigra. Arch Biol Sci. 2017;69(4):679-87.


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How to Cite

Vuksanović V, Kovačević B, Katanić M, Orlović S, Miladinović D. In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra. Arch Biol Sci [Internet]. 2017Oct.18 [cited 2022Aug.9];69(4):679-87. Available from:




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