Variability and relationships among rooting characteristics for white poplar hardwood cuttings


  • Dušan Igić Public Enterprise “Vojvodinašume”, Petrovaradin
  • Milan Borišev University of Novi Sad, Faculty of Science, Department of Biology and Ecology, Novi Sad
  • Dragica Vilotić University of Belgrade, Faculty of Forestry, Belgrade
  • Mirjana Šijačić-Nikolić University of Belgrade, Faculty of Forestry, Belgrade
  • Mirjana Ćuk University of Novi Sad, Faculty of Science, Department of Biology and Ecology, Novi Sad
  • Miloš Ilić University of Novi Sad, Faculty of Science, Department of Biology and Ecology, Novi Sad
  • Branislav Kovačević Institute of Lowland Forestry and Environment, Novi Sad


vegetative propagation, hardwood cutting, plant nursery, Populus alba


Paper description:

  • The hard-to-root property of white poplar hardwood cuttings is one of the principal problems in white poplar nursery production.
  • This research analyses vegetative propagation by hardwood cuttings of four genotypes of white poplar and the variability of 14 morphometric characteristics of cuttings’ rooting, the relation between them and the effect of various technological treatments formed as a combination of applications of powder formulations of indole butyric acid and foliar treatment with urea solution.
  • This study points to significant differences in the rooting and survival of cuttings of white poplar genotypes, but weaker disparities between examined technological and interaction treatments.

Abstract: White poplar is a key species in wood production and afforestation, but also very important for biodiversity preservation and habitat improvement. Vegetative propagation from hardwood cuttings is the most desirable method of white poplar breeding. The present research analyzed the effect of various technological treatments on four white poplar clones. Selected treatments were powder formulations of indole butyric acid (IBA) and foliar treatment with urea. The variability of 14 morphometric characteristics on the rooting of cuttings was analyzed, as well as relationships between them. Differences in analyzed morphometric characteristics depended on genotype, while the effect of the examined technological treatments was detected only by Fisher’s least significant difference (LSD) test. Significant variability between genotypes was found for traits describing rooting at the basal cut (R0 and R0p), the number of leaves (LN), the total number of roots (TRN), and cutting survival in the first part of the growing season (SURV07). Application of IBA had significant stimulating effects on rooting at the lower part of cuttings (R05) compared to controls. Variability between genotypes after foliar treatment with urea was not detected. The examined parameters were grouped into 5 groups by principal component analysis, where shoot traits and traits that describe rooting at the basal cut were in the same group with total number of roots, and cutting survival (SURV07) was in a separate group. These results suggest a need for further testing of the characteristics of hardwood cuttings in white poplars. More efficient clone technology is needed, using auxins and/or nitrogen fertilization designed to suit the specific requirements of particular clones.

Received: January 14, 2020; Revised: January 28, 2020; Accepted: February 20, 2020; Published online: February 24, 2020

How to cite this article: Igić D, Borišev M, Vilotić D, Šijačić-Nikolić M, Ćuk M, Ilić M, Kovačević B. Variability and relationships among rooting characteristics for white poplar hardwood cuttings . Arch Biol Sci. 2020;72(2):153-63.


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Rončević S, Andrašev S, Ivanišević P, Kovačević B, Novčić Z. Effect of variability of alluvial soil properties on growth of white poplars Populus alba L. cl. L-12. In: Rakonjac Lj, editor. Proceedings of International Scientific Conference „Forest in future: Sustainable Use, Risks and Challenges“; 2012 Oct 4-5; Belgrade, Serbia. Belgrade: Institute of Forestry; 2012. p. 469-75.

Vuksanović V, Kovačević B, Kebert M, Katanić M, Pavlović L, Kesić L, Orlović S. Clone specificity of white poplar (Populus alba L.) acidity tolerance in vitro. Fresenius Environmental Bulletin. 2019;28 (11):8307-13.

Dimitri L, Halupa L. III-2: Populus alba L. 1753. In: Roloff A, Weisgerber UM, Lang B, Stimm P, Schütt P, editors. Enzyklopädie der Holzgewächse: Handbuch und Atlas der Dendrologie. Weinheim: Wiley-Vch Verlag; 2013. p. 1-17.

Kovačević B, Orlović S, Pekeč S, Katanić M, Stojnić S. The effect of genotype and date of preparation on rooting of white poplar cuttings. In: Ristić R, Medarević M., Popović Z, editors. Proceedings of First Serbian Forestry “Future with forest”; 2010 Nov 11-13; Belgrade, Serbia. Belgrade: Faculty of Forestry; 2010. p. 306-11.

Abdeldayem M. Evaluation and genetic identification of some Populus species by using polyacrylamide gel electrophoresis separation of total soluble proteins. In: Isebrands JG, Richardson J, editors. General Technical Report NC-215 of 21st session of the International Poplar Commission (IPC 2000) “Poplar and Willow Culture: Meeting the Needs of Society and the Environment”; 2000 Sept 24-28; Vancouver, Washington. St. Paul, Minnesota: North Central Research Station, USDA Forest Service; 2000. p. 1.

Wright W. Introduction to Forest Genetics. New York: Academic Press; 1976. 463 p.

Kovačević B, Guzina V, Andrašev S. Varijabilnost topola u pogledu sposobnosti za ožiljavanje njihovih reznica od pruta. Topola. 2002;169/170:23-36.

Kovačević B, Guzina V, Kraljević-Balalić M, Ivanović M, Nikolić-Đorić E. Evaluation of early rooting traits of eastern cottonwood that are important for selection tests. Silvae Gen. 2008;57(1):13-21.

Smith G, Wareing F. The distribution of latent root primordia in stems of Populus x robusta and factors affecting the emergence of preformed roots from cuttings. Forestry. 1972;45:197-209.

Kovačević B, Orlović S, Pap P, Katanić M, Dabić S. Efekat primene praškastih formulacija sa kobalt-hloridom i indol-buternom kiselinom na ožiljavanje drvenastih reznica bele topole. Topola. 2014;193/194:117-27. Serbian.

Guzina V, Božić J, Tomović Z. Poplars of the Leuce Duby section (White poplars and aspens). In: Guzina V, editor. Poplars and willows in Yugoslavia. Novi Sad: Poplar Research Institute; 1986. p. 74-85.

Guzina V, Tomović Z. Mogućnosti primene metoda kulture tkiva u oplemenjivanju topola. Topola. 1989;155/156:47-56. Serbian.

Orlović S, Kovačević B, Pilipović A, Galić Z. Selekcija i hibridizacija topola iz sekcije Leuce Duby. Radovi Inst Topolarstvo. 2003;28:15- 28.

Fege S. The practice and physiological basis of collecting, storing and planting Populus hardwood cuttings. In: Hansen EA, editor. Intensive plantation culture: 12 years research. General Technical Report. St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Forest Experiment Station; 1983. p. 1-11.

Pregitzer S, Friend L. The structure and function of Populus root Systems. In: Stettler E, Bradshow D; Heilman E, Hinckley M, editors. Biology of Populus and its implications for management and conservation. Ottawa: NRC Research Press; 1996. p. 331-54.

Kovačević B. Genetička divergentnost obrazovanja vegetativnih organa crnih topola (sekcija Aigeiros Duby) [dissertation]. Novi Sad: Faculty of Agriculture, University of Novi Sad; 2003. 173 p. Serbian.

Kovačević B, Rončević S, Miladinović D, Ivanišević P, Katanić M. Early shoot and root growth dynamics as indicators for the survival of black poplar cuttings. New Forests. 2009;38:177-85.

De Almeida R, Aumond M, Da Costa T, Schwambach J, Ruedell M, Correa R, Fett-Neto AG. Environmental control of adventitious rooting in Eucalyptus and Populus cuttings. Trees. 2017;31:1377-90

Kovačević B, Igić D. Effect of early preparation and slope aspect on survival and growth of white poplar rooted cuttings. Topola. 2018;201/202:117-26.

Bellini C, Pacurar I, Perrone I. Adventitious roots and lateral roots: similarities and differences. Ann Rev Plant Biol. 2014;65:639-66.

Zhao X, Zheng H, Li S, Yang C, Jiang J, Liu G. The rooting of poplar cuttings: a review. New Forests. 2014;45:21-34.

Pacurar I, Perrone I, Bellini C. Auxin is a central player in the hormone cross-talks that control adventitious rooting. Physiologia Plantarum. 2014;151:83-96.

Nonić M, Kerkez-Janković I, Aleksić J, Igić D, Šijačić-Nikolić M. Variability of white poplar clones in a nursery trial. Bull Faculty Forestry. 2019;120:131-50.

Zhang B, Tong C, Yin T, Zhang X, Zhuge Q, Huang M, Wang M, Wu R. Detection of quantitative trait loci influencing growth trajectories of adventitious roots in Populus using functional mapping. Tree Genet Gen. 2009;5(3):539-52.

Sun P, Jia H, Zhang Y, Li J, Lu M, Hu J. Deciphering genetic architecture of adventitious root and related shoot traits in Populus using QTL mapping and RNA-Seq data. Int J Mol Sci. 2019;20(24):6114.

Eggens L, Lougheed C, Hilton J. Rooting of hardwood cuttings of boleana poplar. Can J Plant Sci.1972;52:599-604.

Kovačević B, Miladinović D, Orlović S, Tomović Z, Rončević S, Poljaković-Pajnik L. Effect of leaf treatment with cobalt chloride on adventitious rooting of Cottonwood (Populus deltoides Bartr. ex Marsh) cuttings. Prop Orn Plants. 2012;12(1):52-7.

Mironova V, Omelyanchuk N, Yosiphone G, Fadeev S, Kolchanov N, Mjolsness E, Likhoshvai V. A plausible mechanism for auxin patterning along the developing root. BMC Sys Biol. 2010;4:98.

Harfouche A, Baoune N, Merazga H. Main and interaction effects of factors on softwood cutting of white poplar (Populus alba L.). Silvae Gen. 2007;56:287-94.

Rogg LE, Lasswell J, Bartel B. A gain-of-function mutation in IAA28 suppresses lateral root development. Plant Cell. 2001;13:465-80

Druege U, Zerche S, Kadner R. Nitrogen- and storage-affected carbohydrate partitioning in highlight-adapted Pelargonium cuttings in relation to survival and adventitious root formation under low light. Ann Bot. 2004;94:831-42

Zerche S, Druege U. Nitrogen content determines adventitious rooting in Euphorbia pulcherrimaunder adequate light independently of pre-rooting carbohydrate depletion of cuttings. Sci Hort. 2009;121:340-7

Gonin M, Bergougnoux V, Nguzen TD, Gantet P, Champion A. What makes adventitious roots? Plants. 2019;8:240.

Imada S, Yamanaka N, Tamai S. Fine-root growth, fine root mortality, and leaf morphological change of Populus alba in response to fluctuating water tables. Trees. 2010;24(3):499-506.

Kovačević B, Orlović S, Ivanović I, Čobanović K, Nikolić-Dorić E, Katanić M, Galović V. Relationship among eastern cottonwood genotypes according to early rooting traits. Genetika. 2011;43(2):307-20.

Sellmer JC, McCown BH, Haissig BE. Shoot culture dynamics of six Populus clones. Tree Physiol. 1989;5(2):219-27.




How to Cite

Igić D, Borišev M, Vilotić D, Šijačić-Nikolić M, Ćuk M, Ilić M, Kovačević B. Variability and relationships among rooting characteristics for white poplar hardwood cuttings. Arch Biol Sci [Internet]. 2020Jul.1 [cited 2022Jul.5];72(2):153-6. Available from:




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