Prevalence and genetic variability of Plesiomonas shigelloides in temperate climate surface waters of the Pannonian Plain

Authors

  • Milivoje Petrušić University of Novi Sad, Faculty of Sciences
  • Dragana Obreht Vidaković Department of Forest and Conservation Sciences, University of British Columbia, 3027-2424 Main Mall, Vancouver
  • Sava Lazić Department of Virology, Scientific Veterinary Institute ‘‘Novi Sad’’, Rumenački put 20, 21000 Novi Sad
  • Dragan Radnović Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21000 Novi Sad
  • Petar Knežević Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21000 Novi Sad

Keywords:

Plesiomonas shigelloides, surface waters, temperate climate, isolation, RAPD-PCR

Abstract

Plesiomonas shigelloides, a Gram-negative bacterium and the causative agent of intestinal diseases and extraintestinal infections in humans and animals, is most frequently found in aquatic environments in tropical or subtropical areas. The present study was designed to establish the prevalence and genetic variability of P. shigelloides in surface waters (lakes, rivers, ponds, inlets and canals) located in a temperate climate zone, namely the Pannonian Plain of the northern part of Serbia and southern part of Hungary. The strains were isolated directly by plating samples on inositol-brilliant green-bile agar with neutral red or phenol red as indicators. Our results indicate that phenol red effectively facilitates differentiation of P. shigelloides from other bacteria. A number of samples were enriched using alkaline peptone water broth, peptone inositol-bile broth and tetrathionate broth. The recovery of the isolates was more successful with the first medium. Out of a total of 51 water samples collected from 28 different locations, 22 samples (43.1%) were found positive for P. shigelloides. Among the 37 isolated strains, 34 were from lakes (Šatrinci, Ludaš, Panonija, Krivaja, Pecs, Kapetanski rit, Pavlovci, Kovácsszénája, Dobrodol, Vranjaš, Borkovac, Hermann Ottó, Sot, Šelevrenac, Zobnatica, Palić, Orfüí, Jarkovci, Čonoplja) and 3 were from rivers (Danube, Sava). The strains were identified by phenotypic characteristic or by the VITEK2 system and confirmed by PCR using 23S rRNA species-specific oligos. The strains showed a high genetic variability, displaying a variety of RAPD profiles. Our results reveal for the first time a high prevalence of genetically diverse P. shigelloides populations in surface waters located in the temperate climate of central and southeastern Europe.

https://doi.org/10.2298/ABS170530029P

Received: May 30, 2017; Revised: July 17, 2017; Accepted: July 26, 2017; Published online: August 24, 2017

How to cite this article: Petrušić M, Obreht Vidaković D, Lazić S, Radnović D, Knežević P. Prevalence and genetic variability of Plesiomonas shigelloides in temperate climate surface waters of the Pannonian Plain. Arch Biol Sci. 2018;70(1):99-108.

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2018-03-13

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Petrušić M, Obreht Vidaković D, Lazić S, Radnović D, Knežević P. Prevalence and genetic variability of Plesiomonas shigelloides in temperate climate surface waters of the Pannonian Plain. Arch Biol Sci [Internet]. 2018Mar.13 [cited 2024Mar.28];70(1):099-108. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/1828

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