Assessment of genetic diversity within the Merodon ruficornis species group (Diptera: Syrphidae) by RAPD analysis


  • Andrijana Andrić University of Novi Sad, BioSensе Institute, Dr Zorana Đinđića 1, 21000 Novi Sad
  • Nataša Kočiš Tubić University of Novi Sad, Faculty of Sciences
  • Mihajla Đan University of Novi Sad, Faculty of Sciences
  • Ante Vujić University of Novi Sad, Faculty of Sciences
  • Dragana Obreht Vidaković University of Novi Sad, Faculty of Sciences


flower flies, genetic diversity, hoverflies, Merodon, RAPD


As one of the most distinct groups in the hoverfly genus Merodon, the monophyletic ruficornis species group has been the focus of several studies using different approaches. Molecular methods have shown incongruences between morphological and molecular data. In the present study, we investigated four species of the Merodon ruficornis group (i.e. M. loewi, M. armipes, M. papillus and M. hoplitis) with the aim of detecting intra- and interspecific genetic diversity, and we examined the usefulness of random amplified polymorphic DNA (RAPD) in an integrative taxonomic approach to species delimitation. Analysis of Nei’s genetic variation over all loci showed that genetic diversity for the analyzed Merodon species was h=0.24. Based on UPGMA, PCoA and Bayesian clustering analyses, our results clearly differentiated four groups that correspond to the four morphologically-defined Merodon species. Among the analyzed species, M. armipes and M. hoplitis showed the lowest level of genetic divergence; M. loewi was clearly separated from both M. armipes and M. papillus. Based on our data, we propose the use of RAPD-PCR as an additional tool for resolving taxonomic problems within Merodon.

Received: July 29, 2016; Revised: November 15, 2016; Accepted: December 5, 2016; Published online: December 8, 2016

How to cite: Andrić A, Kočiš-Tubić N, Đan M, Vujić A, Obreht Vidaković D. Assessment of genetic diversity within the Merodon ruficornis species group (Diptera: Syrphidae) by RAPD analysis. Arch Biol Sci. 2017;69(3):553-60.


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Author Biographies

Nataša Kočiš Tubić, University of Novi Sad, Faculty of Sciences

Department of Biology and Ecology

Mihajla Đan, University of Novi Sad, Faculty of Sciences

Department of Biology and Ecology

Ante Vujić, University of Novi Sad, Faculty of Sciences

Department of Biology and Ecology

Dragana Obreht Vidaković, University of Novi Sad, Faculty of Sciences

Department of Biology and Ecology


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How to Cite

Andrić A, Kočiš Tubić N, Đan M, Vujić A, Obreht Vidaković D. Assessment of genetic diversity within the Merodon ruficornis species group (Diptera: Syrphidae) by RAPD analysis. Arch Biol Sci [Internet]. 2017Aug.11 [cited 2022Aug.9];69(3):553-60. Available from: