Variations in leaf gas exchange, chlorophyll fluorescence and membrane potential of Medicago sativa root cortex cells exposed to increased salinity: The role of the antioxidant potential in salt tolerance

Authors

  • Mohamed Farissi Laboratory of Biotechnology and Sustainable Development of Natural Resources, Polydisciplinary Faculty, Sultan Moulay Slimane University, PO Box: 592, Beni-Mellal, 23000 http://orcid.org/0000-0003-2853-9115
  • Mohammed Mouradi 1. Unit of Biotechnology and Agro-physiology of Symbiosis, Faculty of Sciences and Techniques, PO. Box 549, Gueliz 40000 Marrakesh; 2.Unit of Plant Breeding, National Institute for Agronomic Research (INRA), PO. Box 533, Gueliz 40000, Marrakesh http://orcid.org/0000-0002-6216-9683
  • Abdelaziz Bouizgaren Unit of Plant Breeding, National Institute for Agronomic Research (INRA), PO. Box 533, Gueliz 40000, Marrakesh http://orcid.org/0000-0003-3593-720X
  • Cherki Ghoulam Unit of Biotechnology and Agro-physiology of Symbiosis, Faculty of Sciences and Techniques, PO. Box 549, Gueliz 40000 Marrakesh http://orcid.org/0000-0002-3138-249X

Keywords:

alfalfa, salinity, leaf exchange, membrane potential, antioxidant enzymes

Abstract

Paper description:

  • Salinity is one of the most serious agricultural problems that adversely affects growth and productivity of pasture crops.
  • The effects of salinity on some ecophysiological and biochemical criteria associated with salt tolerance were assessed in two Moroccan alfalfa (Medicago sativa L.) populations, Taf 1 and Tata in a hydro-aeroponic system.
  • The Tata population was more tolerant to high salinity (200 mM NaCl) and its tolerance was associated with the ability of plants to maintain adequate levels of the studied parameters and their ability to overcome oxidative stress by the induction of antioxidant enzymes, guaiacol peroxidase, catalase and superoxide dismutase.


Abstract: Salinity is one of the most serious agricultural problems that adversely affects growth and productivity of pasture crops such as alfalfa. In this study, the effects of salinity on some ecophysiological and biochemical criteria associated with salt tolerance were assessed in two Moroccan alfalfa (Medicago sativa L.) populations, Taf 1 and Tata. The experiment was conducted in a hydro-aeroponic system containing nutrient solutions, with the addition of NaCl at concentrations of 100 and 200 mM. The salt stress was applied for a month. Several traits in relation to salt tolerance, such as plant dry biomass, relative water content, leaf gas exchange, chlorophyll fluorescence, nutrient uptake, lipid peroxidation and antioxidant enzymes, were analyzed at the end of the experiment. The membrane potential was measured in root cortex cells of plants grown with or without NaCl treatment during a week. The results indicated that under salt stress, plant growth and all of the studied physiological and biochemical traits were significantly decreased, except for malondialdehyde and H2O2 contents, which were found to be increased under salt stress. Depolarization of membrane root cortex cells with the increase in external NaCl concentration was noted, irrespective of the growth conditions. The Tata population was more tolerant to high salinity (200 mM NaCl) and its tolerance was associated with the ability of plants to maintain adequate levels of the studied parameters and their ability to overcome oxidative stress by the induction of antioxidant enzymes, such as guaiacol peroxidase, catalase and superoxide dismutase.

https://doi.org/10.2298/ABS171019001F

Received: October 19, 2017; Revised: December 1, 2017; Accepted: December 15, 2017; Published online: January 8, 2018

How to cite this article: Farissi M, Mouradi M, Farssi O, Bouizgaren A, Ghoulam C. Variations in leaf gas exchange, chlorophyll fluorescence and membrane potential of Medicago sativa root cortex cells exposed to increased salinity: The role of the antioxidant potential in salt tolerance. Arch Biol Sci. 2018;70(3):…

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2018-08-20

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Farissi M, Mouradi M, Bouizgaren A, Ghoulam C. Variations in leaf gas exchange, chlorophyll fluorescence and membrane potential of Medicago sativa root cortex cells exposed to increased salinity: The role of the antioxidant potential in salt tolerance. Arch Biol Sci [Internet]. 2018Aug.20 [cited 2022May29];70(3):413-2. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2279

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