Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles

Authors

  • Marko B. Mitrović Department of Radiology and Radiation Hygiene, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobođenja 18, 11000 Belgrade http://orcid.org/0000-0002-0416-0103
  • Nikola R. Tatalović Department of Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-0745-8845
  • Aleksandra L. Nikolić-Kokić Department of Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-1116-2035
  • Olivera F. Ciraj-Bjelac Radiation Protection Department, Vinča Institute of Nuclear Sciences, University of Belgrade, Mike Petrovića Alasa 12-14, 11351 Belgrade http://orcid.org/0000-0001-9577-011X
  • Nikola E. Krstić Department of Radiology and Radiation Hygiene, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobođenja 18, 11000 Belgrade http://orcid.org/0000-0001-7326-6262
  • Zorana S. Oreščanin-Dušić Department of Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-2772-0134
  • Dragana Ž. Krstić Department of Physics, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac http://orcid.org/0000-0002-3517-9210
  • Zoran M. Jovanović Department of Physics, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac http://orcid.org/0000-0002-0898-8960
  • Duško P. Blagojević Department of Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0001-6338-2833
  • Mirjana V. Lazarević-Macanović Department of Radiology and Radiation Hygiene, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobođenja 18, 11000 Belgrade http://orcid.org/0000-0003-0668-184X

Keywords:

computed tomography, oxidative stress, antioxidant enzymes, testicles, rabbit

Abstract

Paper description:

  • There is no literature data on the influence of diagnostic doses of x-radiation during computed tomography (CT) on the oxidative status in testicles.
  • Our results showed that CT provoked a response at the level of antioxidant enzymes in rabbit testicles which was time- and dose-dependent, with a dose threshold of about 29.2 mGy, and predominantly under the influence of the applied current.
  • Our results present novel information about the influence of CT on the cellular antioxidant process.

Abstract: In recent years, computed tomography (CT) has become very common in veterinary medicine. It is well known that testicles are organs with high radiosensitivity and their function can be impaired even after exposure to low radiation doses. In this work, we calculated the absorbed radiation doses after CT was performed with different voltage/current levels and correlated it with the activity of antioxidant enzymes in rabbit testicles. Two hours after CT, the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) were increased in the testicles of animals that received an absorbed dose of 29.2 mGy. The same changes, along with elevated glutathione reductase (GR) activity, were observed after 7 days in animals that received the highest absorbed dose (46.3 mGy). It would appear that absorbed doses above 27.8 mGy provoked the antioxidant reaction but the time scale of the reaction was dose-dependent. Examination of the obtained results revealed that the main denominator of CT influence was a higher current. Our results suggest that CT influences the antioxidant status in rabbit testicles. The changes in antioxidant enzyme activities were dose- and time-dependent and influenced by the applied current.

https://doi.org/10.2298/ABS180413029M

Received: April 13, 2018; Revised: May 23, 2018; Accepted: June 12, 2018; Published online: June 15, 2018

How to cite this article: Mitrović MB, Tatalović NR, Nikolić-Kokić AL, Ciraj-Bjelac OF, Krstić NE, Oreščanin-Dušić ZS, Krstić DŽ, Jovanović ZM, Blagojević DP, Lazarević-Macanović MV. Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles. Arch Biol Sci. 2018;70(4):675-80.

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Author Biography

Aleksandra L. Nikolić-Kokić, Department of Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade


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Published

2018-12-04

How to Cite

1.
Mitrović MB, Tatalović NR, Nikolić-Kokić AL, Ciraj-Bjelac OF, Krstić NE, Oreščanin-Dušić ZS, Krstić D Ž., Jovanović ZM, Blagojević DP, Lazarević-Macanović MV. Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles. Arch Biol Sci [Internet]. 2018Dec.4 [cited 2022Aug.9];70(4):675-80. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2841

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