Evaluation of anticancer and antimicrobial activities of the Polygonum maritimum ethanol extract

Authors

  • Marina Jovanović University of Belgrade – Faculty of Biology, Studentski trg 16, 11000 Belgrade
  • Tatjana Srdić-Rajić Institute of Oncology and Radiology, Pasterova 14, 11000 Belgrade
  • Emilija Svirčev University of Novi Sad – Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection, Trg Dositeja Obradovića 3, 21000 Novi Sad
  • Nebojša Jasnić University of Belgrade – Faculty of Biology, Studentski trg 16, 11000 Belgrade
  • Biljana Nikolić University of Belgrade – Faculty of Biology, Studentski trg 16, 11000 Belgrade
  • Svetlana Bojić University of Belgrade – Faculty of Biology, Studentski trg 16, 11000 Belgrade
  • Tatjana Stević Institute for Medicinal Plants Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade
  • Jelena Knežević-Vukčević University of Belgrade – Faculty of Biology, Studentski trg 16, 11000 Belgrade
  • Dragana Mitić-Ćulafić University of Belgrade – Faculty of Biology, Studentski trg 16, 11000 Belgrade

Keywords:

P. maritimum, antifungal effect, synergism with doxorubicin, flow cytometry, antioxidant enzymes

Abstract

Paper description:

  • Polygonum plants possess different biological activities such as antioxidant, antimicrobial, anti-inflammatory, anticancer, antidiabetic and neuroprotective effects.
  • To the best of our knowledge, the antifungal effect of the Polygonum maritimum ethanol extract has not been evaluated. We examined for the first time the potential of the extract to modulate the effect of doxorubicin on hepatoma cell viability, apoptosis and cell cycle arrest and expression of antioxidant enzymes.
  • Our study provides new insight into the potential application of P. maritimum extract as a natural fungicide and as a potential adjuvant in chemotherapy against hepatocarcinoma.

Abstract: Polygonum maritimum is a traditional herbal remedy that produces abundant flavonoid secondary metabolites. The ethanol extract of P. maritimum aerial parts (POM) was chemically characterized and tested for antimicrobial properties and cytotoxicity. Results of LC-MS/MS analysis showed high contents of gallic acid, epigallocatechin gallate and catechin, and significant amounts of quercetin-3-O-galactoside and quercetin-3-O-glucoside. Evaluation of the antifungal properties revealed that POM induced notable growth inhibition of Alternaria alternata (34.3%), Penicillium spp. (30.6%), Fusarium semitectum (20.2%) and Aspergillus spp. (19.6%). Evaluation of cytotoxicity against human hepatoma HepG2 cells included monitoring the effects of both POM alone and its combination with cytostatic doxorubicin (Dox). Cell viability, apoptosis and cell cycle distribution and the expression of antioxidant enzymes (superoxide-dismutases SOD1 and SOD2 and catalase) were determined. A dose-dependent decrease in cell viability was detected, but a remarkably stronger effect was obtained when POM and Dox were applied in combination as compared to individual treatments. IC50 values were determined to be 393 μg/mL (POM) and 2.24 μg/mL (Dox) in combination, but 1153 μg/mL (POM) and 12.56 μg/mL (Dox) in a single treatment. The value of the Loewe index, determined for IC50,was notably lower than 1 (LI=0.51), clearly indicating synergism of POM and Dox. Additionally, POM and POM +Dox induced early/late apoptosis and G2/M cell cycle arrest. Furthermore, POM increased, while Dox decreased the expression levels of SODs and catalase. The obtained results encourage further examination of the potential use of POM in modern phytotherapy.

https://doi.org/10.2298/ABS180423028J

Received: April 23, 2018; Revised: June 6, 2018; Accepted: June 6, 2018; Published online: June 12, 2018

How to cite this article: Jovanović M, Srdić-Rajić T, Svirčev E, Jasnić N, Nikolić B, Bojić S, Stević T, Knežević-Vukčević J, Mitić-Ćulafić D. Evaluation of anticancer and antimicrobial activities of the Polygonum maritimum ethanol extract. Arch Biol Sci. 2018;70(4):665-73.

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Published

2018-12-04

How to Cite

1.
Jovanović M, Srdić-Rajić T, Svirčev E, Jasnić N, Nikolić B, Bojić S, Stević T, Knežević-Vukčević J, Mitić-Ćulafić D. Evaluation of anticancer and antimicrobial activities of the Polygonum maritimum ethanol extract. Arch Biol Sci [Internet]. 2018Dec.4 [cited 2022Dec.8];70(4):665-73. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2871

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