Element accumulation capacity of Vaccinium myrtillus from Montenegro: Comparison of element contents in water and ethanol extracts of bilberry plant parts

Authors

Keywords:

Vaccinium myrtillus, wild bilberry, extraction coefficients, ethanol extracts, daily metal intake

Abstract

Paper description:

  • Bilberry (Vaccinium myrtillus L., Ericaceae) and its extracts are used in traditional medicine. While research has focused on the health benefits of plant flavonoids, the element content is of potentialy great importance.
  • This study provides information on which part of the bilberry (roots, stems, leaves or fruits) accumulates which elements and on how the metals taken up from the soil might be translocated from the roots to the above-ground parts of the plant.
  • This study provides new findings on element distribution in bilberry and gives insight into the effect of the method of bilberry extract preparation on the element content.

Abstract: Bilberry (Vaccinium myrtillus L., Ericaceae) is a rich source of different mineral elements that are important as essential trace elements, or that can be potentially toxic, depending on their concentration. The contents of sixteen elements: the major (Al, Ca, Fe, K and Mg) and trace elements (As, Ba, Cd, Co, Cu, Cr, Mn, Ni, Pb, Sr and Zn) in roots, stems, leaves and fruits of bilberry and the corresponding soil substrate were determined in order to investigate element mobility and availability in soil. Soil was subjected to sequential extraction procedure as proposed by the Community Bureau of Reference in order to fractionate acid-soluble/exchangeable, reducible, oxidizable and residual fractions. Analysis of water and ethanol extracts of bilberry aerial parts revealed a significant transfer of elements during the extraction procedure, with corresponding extraction coefficient values of up to 95.12%. Results showed that bilberry fruits could serve as a good dietary source of essential elements for humans, especially Mn and Cu.

https://doi.org/10.2298/ABS181004056B

Received: October 4, 2018; Revised: November 28, 2018; Accepted: November 30, 2018; Published online: December 14, 2018

How to cite this article: Brašanac-Vukanović S, Tadić VM, Blagojević N, Vukašinović-Pešić V, Đurđić S, Stanković M, Mutić J. Element accumulation capacity of Vaccinium myrtillus from Montenegro: Comparison of element contents in water and ethanol extracts of bilberry plant parts. Arch Biol Sci. 2019;71(1):145-57.

Downloads

Download data is not yet available.

References

Chu W, Cheung SCM, Lau RAW, Benzie IFF. Bilberry (Vaccinium myrtillus L.). In: Benzie IFF, Wachtel-Galor S, editors. Herbal medicine biomolecular and clinical aspects. Boca Raton: CRC Press; 2011. p. 55-73.

Može S, Polak T, Gašperlin L, Koron D, Vanzo A, Poklar Ulrih N, Abram V. Phenolics in Slovenian bilberries (Vaccinium myrtillus L.) and blueberries (Vaccinium corymbosum L.). J Agric Food Chem. 2011;59(13):6998-7004.

Brasanac-Vukanovic S, Mutic J, Stankovic DM, Arsic I, Blagojevic N, Vukasinovic-Pesic V, Tadic VM. Wild Bilberry (Vaccinium myrtillus L., Ericaceae) from Montenegro as a Source of Antioxidants for Use in the Production of Nutraceuticals. Molecules. 2018;23(8):1864-83.

Kostić D, Mitić S, Zarubica A, Mitić M, Veličković J, Randjelović S. Content of trace metals in medicinal plants and their extracts. Hem Ind. 2011;65(2):165-70.

Demczuk M, Garbiec K. Heavy metals in edible fruits. A case study of bilberry Vaccinium myrtillus L. Environ Prot Nat Resour. 2009;40:307-12.

Gallaher RN, Gallaher K, Marshall AJ, Marshall AC. Mineral analysis of ten types of commercially available tea. J Food Compos Anal. 2006;19:53-7.

Kandziora-Ciupa M, Ciepał R, Nadgórska-Socha A, Barczyk G. A comparative study of heavy metal accumulation and antioxidant responses in Vaccinium myrtillus L. leaves in polluted and non-polluted areas. Environ Sci Pollut Res. 2013;20(7):4920-32.

Kozanecka T, Chojnicki J, Kwasowski W. Content of heavy metals in plant from pollution-free regions. Polish J Environ Stud. 2002;11(4):395-9.

Parzych A. The heavy metal content of soil and shoots of Vaccinium myrtillus L. in the Słowiński national park. For Res Pap. 2014;75(3):217-24.

Reimann C, Koller F, Frengstad B, Kashulina G, Niskavaara H, Englmaier P. Comparison of the element composition in several plant species and their substrate from a 1500000-km2 area in Northern Europe. Sci Total Environ. 2001;278(1–3):87-112.

Skesters A, Kleiner D, Blázovics A, May Z, Kurucz D, Szentmihályi K. Mineral element content and antioxidant capacity of some Latvian berries. Eur Chem Bull. 2013;3(1):98-101.

Alvarez JM, Lopez-Valdivia LM, Novillo J, Obrador A, Rico MI. Comparison of EDTA and sequential extraction tests for phytoavailability prediction of manganese and zinc in agricultural alkaline soils. Geoderma. 2006;132(3-4):450-63.

Kubová J, Matúš P, Bujdoš M, Hagarová I, Medved’ J. Utilization of optimized BCR three-step sequential and dilute HCl single extraction procedures for soil–plant metal transfer predictions in contaminated lands. Talanta. 2008;75(4):1110-22.

Zimmerman AJ, Weindorf DC. Heavy metal and trace metal analysis in soil by sequential extraction: a review of procedures. Int J Anal Chem. 2010;2010(3-4):387803.

Stefanović V, Trifković J, Mutić J, Tešić Ž. Metal accumulation capacity of parasol mushroom (Macrolepiota procera) from Rasina region (Serbia). Environ Sci Pollut Res. 2016;23(13):13178-90.

Davidson CM, Duncan AL, Littlejohn D, Ure AM, Garden LM. A critical evaluation of the three-stage BCR sequential extraction procedure to assess the potential mobility and toxicity of heavy metals in industrially-contaminated land. Anal Chim Acta. 1998;363(1):45-55.

Rauret G, López-Sánchez JF, Sahuquillo A, Barahona E, Lachica M, Ure AM, Davidson CM, Gomez A, Lück D, Bacon J, Yli-Halla M, Muntau H, Quevauviller P. Application of a modified BCR sequential extraction (three-step) procedure for the determination of extractable trace metal contents in a sewage sludge amended soil reference material (CRM 483), complemented by a three-year stability study of acetic acid and EDTA extractable metal content. J Environ Monit. 2000;2(3):228-33.

Mossop KF, Davidson CM. Comparison of original and modified BCR sequential extraction procedures for the fractionation of copper, iron, lead, manganese and zinc in soils and sediments. Anal Chim Acta. 2003;478(1):111-8.

Rosłon W, Osińska E, Ewelina Pióro-Jabrucka E, Grabowska A. Morphological and chemical variability of wild populations of bilberry (Vaccinium myrtillus L.). Polish J Environ Stud. 2011;20(1):237-43.

Szymczycha-Madeja A, Welna M, Pohl P. Elemental analysis of teas and their infusions by spectrometric methods. Trac-Trend Anal Chem. 2012;35:165-81.

European Economic Community (EEC). Commission Directive 2008/100/EC. Amending Council Directive 90/496/EEC on nutrition labelling for food stuffs as regards recommended daily allowances, energy conversion factors and definitions. Off J Eur Commun. 2008;L285:9.

Kabata-Pendias A. Trace Elements in soils and plants. 4th ed. Boca Raton: CRC Press; 2011. 520 p.

Sungur A, Soylak M, Ozcan H. Investigation of heavy metal mobility and availability by the BCR sequential extraction procedure: relationship between soil properties and heavy metals availability. Chem Speciat Bioavailab. 2014;26(4):219-30.

Rulebook on permissible quantities of dangerous and harmful substances in soil and water for irrigation and methods of their testing. Official Gazette of the Republic of Serbia. 1994;23.

Jain CK. Metal fractionation study on bed sediments of River Yamuna, India. Water Res. 2004;38(3):569-78.

Chizzola R. Metallic mineral elements and heavy metals in medicinal plants. Med Aromat Plant Biotechnol. 2012;6:39-53.

Ozgen S, Busse SJ, Palta PJ. Influence of root zone calcium on shoot tip necrosis and apical dominance of potato shoot: simulation of this disorder by ethylene glycol tetra acetic acid and prevention by strontium. Hortscience. 2011;46(10):1358-62.

Cataldo DA, Garland TR, Wildung RE. Nickel in plants: I. Uptake kinetics using intact soybean seedlings. Plant Physiol. 1978;62(4):563-5.

Li HF, Gray C, Mico C, Zhao FJ, McGrath SP. Phytotoxicity and bioavailability of cobalt to plants in a range of soils. Chemosphere. 2009;75(7):979-86.

Patel P, Patel NM, Patel PM. Who guidelines on quality control of herbal medicines. Int J Res Ayurveda Pharm. 2011;2(24):1148-54.

Donkora A, Kuranchiea C, Osei-Fosua P, Nyarkoa S, Doamekpora L. Assessment of essential minerals and toxic trace metals in popularly consumed tea products in Ghana, A Preliminary Study. Res J Chem Environ Sci. 2015;3(1):49-55.

Arika WM, Ogola PE, Nyamai DW, Mawia AM, Wambua FK, Kiboi NG, Wambani JR, Njagi SM, Rachuonyo HO, Emmah KO, Lagat RC, Muruthi CW, Abdirahman YA, Agyirifo DS, Ouko RO, Ngugi MP, Njagi ENM. Mineral elements content of selected Kenyan antidiabetic Medicinal Plants. Adv Tech Biol Med. 2015;4(1):1-5.

Khan AR, Awan FR. Metals in the pathogenesis of type 2 diabetes. J Diabetes Metab Disord. 2014;13(1):16.

European Food Safety Authority (EFSA). Dietary exposure to inorganic arsenic in the European population. EFSA J. 2014;12(3): 3597.

European Food Safety Authority (EFSA). Cadmium dietary exposure in the European population. EFSA J. 2012;10(1): 2551.

European Food Safety Authority (EFSA). Scientific opinion on lead in food. EFSA panel on contaminants in the food chain (CONTAM). EFSA J. 2010;8(4): 1570.

Downloads

Published

2019-04-02

How to Cite

1.
Brašanac Vukanović S, Tadić VM, Blagojević N, Vukašinović Pešić V, Đurđić S, Stanković M, Mutić J. Element accumulation capacity of Vaccinium myrtillus from Montenegro: Comparison of element contents in water and ethanol extracts of bilberry plant parts. Arch Biol Sci [Internet]. 2019Apr.2 [cited 2024Apr.19];71(1):145-57. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/3485

Issue

Section

Articles

Most read articles by the same author(s)