Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina
Keywords:retina, EGFR, progenitors, differentiation, postnatal development
- Different levels of Epidermal Growth Factor (EGF) signaling can have different effects on progenitor’s destiny during the embryonic and postnatal retinal development.
- The addition of EGF-receptor into postnatal retinal progenitors ensured the sustained signaling in the presence of the exogenous EGF ligand during the first postnatal week in the model system of the retinal explants (REs).
- The continuous EGF signaling altered postnatal progenitors’ competence restrictions.
- Better understanding of how different levels of EGF signaling can affect progenitor’s competence can elucidate the ways of interfering with the developmental programs and thus facilitate retinal regeneration.
Abstract: Epidermal growth factor (EGF) signaling has been implicated in the regulation of the differentiation and proliferation of retinal progenitors. We assessed how different levels of EGF signaling, achieved either by increasing receptor expression or via addition of the exogenous ligand, or an increase in both, can affect the differentiation of progenitors in the first week of postnatal retinal development in the model system of retinal explants (REs). Proliferating progenitor cells in REs were infected with either the control CLV3/ESR-related peptide family (CLE)-green fluorescent protein (GFP)- or with EGF receptor (EGFR)-GFP-expressing retrovirus, and grown in the control medium or in the presence of exogenous EGF (10 ng/mL). The differentiation of infected cells into Müller glia (Sox9+), rod photoreceptors (rhodopsin+) and horizontal cells (calbindin+) was analyzed. In all the examined conditions, infected cells differentiated into Müller glia and rod photoreceptors that normally develop postnatally. Horizontal cells finished their development during the embryonic stages and progenitors infected with control-GFP virus did not differentiate into GFP+/calbindin- in either control or EGF-supplemented medium, however, cells infected with EGFR-GFP differentiated into horizontal cells (GFP+/calbindin+) in both culture conditions. These results imply that altering the levels of EGFR and/or the amount of the EGF ligand can overcome progenitor competence restriction.
Received: June 17, 2019; Revised: August 22, 2019; Accepted: September 4, 2019; Published online: September 5, 2019
How to cite this article: Ivković S, Jovanović-Macura I, Antonijević T, Kanazir S, Henrique D. Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina. Arch Biol Sci. 2019;71(4):711-9.
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