Centaurium erythraea extract reduces redox imbalance and improves insulin expression and secretion in pancreatic β-cells exposed to oxidative and nitrosative stress

Authors

  • Miloš Đorđević Department of Molecular Biology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0001-9951-1932
  • Nevena Grdović Department of Molecular Biology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0003-1504-4578
  • Mirjana Mihailović Department of Molecular Biology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0002-8097-1186
  • Jelena Arambašić Jovanović Department of Molecular Biology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0002-8810-0668
  • Aleksandra Uskoković Department of Molecular Biology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0002-9616-1102
  • Jovana Rajić Department of Molecular Biology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0002-2808-8484
  • Marija Đorđević Department of Molecular Biology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0001-9051-9583
  • Anja Tolić Department of Molecular Biology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0003-4703-6214
  • Danijela Mišić Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0002-5419-0773
  • Branislav Šiler Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0001-5831-6612
  • Goran Poznanović Department of Molecular Biology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0003-0839-7017
  • Melita Vidaković Department of Molecular Biology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0001-8410-6264
  • Svetlana Dinić Department of Molecular Biology, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade http://orcid.org/0000-0002-7612-3117

Keywords:

oxidative stress, nitrosative stress, β-cells, Centaurium erythraea, antioxidant, cytoprotective

Abstract

Paper description:

  • Oxidative stress underlies b-cell damage and a diminished capacity for insulin secretion in diabetes.
  • Previously we have shown that the Centaurium erythraea extract (CEe) exhibits a significant redox potential both in vitroand in an animal model of diabetes.
  • Herein we examined the antioxidant effects of CEe in H2O2-and sodium nitroprusside (SNP)-treated Rin-5F b-cells.
  • CEe attenuated the H2O2- and SNP-induced redox imbalance, contributing to a decrease in DNA, lipid and protein damage and to an increase in cell-viability and insulin expression and secretion.


Abstract: Oxidative stress is one of the major mechanisms that underlies the damage of pancreatic b-cells and defects in insulin secretion in diabetes. As herbal preparations can alleviate oxidative stress through their redox-active secondary metabolites, in this study we investigated the cytoprotective effects of Centaurium erythraea extract (CEe) against H2O2- and SNP-induced oxidative/nitrosative stress in Rin-5F b-cells. The antioxidant activity of CEe and its effect on cell survival and insulin expression/secretion were evaluated. The CEe increased cell viability and ameliorated the disturbance of redox homeostasis in H2O2- and SNP-treated cells by decreasing DNA damage, lipid peroxidation and protein S-glutathionylation. The CEe restored GSH homeostasis in H2O2-treated b-cells and attenuated the SNP-induced disturbance of the GSH/GSSG ratio. The H2O2- and SNP-induced disruption of CAT, GPx, GR, MnSOD and CuZnSOD activities was adjusted by the CEe towards control values, as well as mRNA and protein levels of GPx, MnSOD and CAT. The CEe increased insulin expression/secretion particularly in H2O2-treated b-cells, which was in accordance with the more pronounced antioxidant effect of the CEe observed in H2O2-treated b-cells as compared to SNP-treated cells. These findings support the beneficial effect of the CEe in preventing or slowing down b-cell damage and dysfunction caused by oxidative/nitrosative stress during diabetes development.

https://doi.org/10.2298/ABS200127005D

Received: January 27, 2020; Revised: January 31, 2020; Accepted: January 31, 2020; Published online: February 10, 2020

How to cite this article: Đorđević M, Grdović N, Mihailović M, Arambašić Jovanović J, Uskoković A, Rajić J, Đorđević M, Tolić A, Mišić D, Šiler B, Poznanović G, Vidaković M, Dinić S. Centaurium erythraea extract reduces redox imbalance and improves insulin expression and secretion in pancreatic β-cells exposed to oxidative and nitrosative stress. Arch Biol Sci. 2020;72(1):117-28.

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Published

2020-03-24

How to Cite

1.
Đorđević M, Grdović N, Mihailović M, Arambašić Jovanović J, Uskoković A, Rajić J, Đorđević M, Tolić A, Mišić D, Šiler B, Poznanović G, Vidaković M, Dinić S. Centaurium erythraea extract reduces redox imbalance and improves insulin expression and secretion in pancreatic β-cells exposed to oxidative and nitrosative stress. Arch Biol Sci [Internet]. 2020Mar.24 [cited 2022Jan.19];72(1):117-28. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5029

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