Effects of exogenous salicylic acid on physiological traits and CBF gene expression in peach floral organs under freezing stress
Keywords:peach, floral organ, salicylic acid, freezing stress, physiological trait, CBF
To elucidate the effects of exogenous salicylic acid (SA) treatment on the cold resistance of peach flower, the floral organs of two peach cultivars were treated with 20 mg/L SA and stored at 0°C for observation and sample collection. Water application was the control. After a treatment period, the anther relative water content of the control and SA-treated flowers decreased. The extent of the reduction was greater in the control, suggesting that the SA treatment significantly helped to maintain the anther water content of peach. Analysis of the stigma relative electric conductivity revealed that the SA treatment prevented membrane injury during the low temperature treatment. Additionally, we measured CBF gene expression at low temperature in the petal, stigma and ovary. The expression was markedly upregulated in the cold-treated floral organs. CBF gene expression after SA treatment was higher than in the control when cold conditions continued. These results suggest that the effects of SA on ameliorating the freezing injury to peach floral organs and on enhancing cold tolerance may be associated with the induction of CBF gene.
Received: August 16, 2016; Revised: November 9, 2016; Accepted: December 14, 2016; Published online: January 13, 2017
How to cite this article: Zhang B, Ma R, Guo L, Song Z, Yu M. Effects of exogenous salicylic acid on physiological traits and CBF gene expression in peach floral organs under freezing stress. Arch Biol Sci. 2017;69(4):585-92.
Kasuga M, Liu Q, Miura S, Yamaguchi-Shinozaki K, Shinozaki K. Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat Biotechnol. 1999;17(3):287-91.
Savage JA, Cavender-Bares J. Phenological cues drive an apparent trade-off between freezing tolerance and growth in the family Salicaceae. Ecology. 2013;94(8):1708-17.
Fennell A. Freezing tolerance and injury in grapevines. J Crop Improv. 2004;10(1-2):201-35.
Salazar-Gutiérrez MR, Chaves B, Hoogenboom G. Freezing tolerance of apple flower buds. Sci Hortic. 2016;198:344-51.
Szymajda M, Pruski K, Żurawicz E, Sitarek M. Freezing injuries to flower buds and their influence on yield of apricot (Prunus armeniaca L.) and peach (Prunus persica L.). Can J Plant Sci. 2013;93:191-8.
Reig G, Iglesias I, Miranda C, Gatius F, Alegre S. How does simulated frost treatment affect peach [Prunus persica (L.)] flowers of different cultivars from worldwide breeding programmes? Sci Hortic. 2013;160:70-7.
Duan J, Zhang QB, Lv L, Zhang C. Regional-scale winter-spring temperature variability and chilling damage dynamics over the past two centuries in southeastern China. Clim Dynam. 2012;39(3-4):919-28.
Rodrigo J. Spring frosts in deciduous fruit trees-morphological damage and flower hardiness. Sci Hortic. 2000;85(3):155-73.
Hayat Q, Hayat S, Irfan M, Ahmad A. Effect of exogenous salicylic acid under changing environment: a review. Environ Exp Bot. 2010;68:14-25.
Miura K, Tada Y. Regulation of water, salinity, and cold stress responses by salicylic acid. Front Plant Sci. 2014;5:1-12.
Vlot AC, Dempsey DA, Klessig DF. Salicylic acid, a multifaceted hormone to combat disease. Annu Rev Phytopathol. 2009;47:177-206.
Ananieva EA, Christov KN, Popova LP. Exogenous treatment with salicylic acid leads to increased antioxidant capacity in leaves of barley plants exposed to paraquat. J Plant Physiol. 2004;161;319-28.
Cao S, Hu Z, Zheng Y, Lu B. Synergistic effect of heat treatment and salicylic acid on alleviating internal browning in cold-stored peach fruit. Postharvest Bio Technol. 2010;58:93-7.
Kim Y, Park S, Gilmour SJ, Thomashow MF. Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis. Plant J. 2013;75(3):364-76.
Taşgın E, Atıcı Ö, Nalbantoğlu B, Popova LP. Effects of salicylic acid and cold treatments on protein levels and on the activities of antioxidant enzymes in the apoplast of winter wheat leaves. Phytochemistry. 2006;67(7):710-5.
Hashempour A, Ghasemnezhad M, Ghazvini RF, Sohani MM. The physiological and biochemical responses to freezing stress of olive plants treated with salicylic acid. Russ J Plant Physiol. 2014;61(4):443-50.
Unal BT, Mentis O, Akyol E. Effects of exogenous salicylic acid on antioxidant activity and proline accumulation in apple (Malus domestica L.). Hortic Environ Biote. 2015;56(5):606-11.
Keshavarz H, Sanavy SAMM, Moghadam RSG. Impact of foliar application with salicylic acid on biochemical characters of canola plants under cold stress condition. Not Sci Biol. 2016;8(1):98-105.
Janda T, Szalai G, Rios-Gonzales K, Veisa O Paldi E. Comparative study of frost tolerance and antioxidant activity in cereals. Plant Sci. 2003;164:301-6.
Licausi F, Ohme-Takagi M, Perata P. APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factors: mediators of stress responses and developmental programs. New Phytol. 2013;199(3):639-49.
Akhtar M, Jaiswal A, Taj G, Jaiswal JP, Qureshi MI, Singh NK. DREB1/CBF transcription factors: their structure, function and role in abiotic stress tolerance in plants. J Genet. 2012;91(3):385-95.
Lee YP, Fleming AJ, Koerner C, Meins Jr F. Differential expression of the CBF pathway and cell cycle-related genes in Arabidopsis accessions in response to chronic low-temperature exposure. Plant Bio. 2009;11(3):273-83.
Ma Q, Suo J, Huber DJ, Dong X, Han Y, Zhang Z, Rao J. Effect of hot water treatments on chilling injury and expression of a new C-repeat binding factor (CBF) in ‘Hongyang’ kiwifruit during low temperature storage. Postharvest Bio Technol. 2014;97:102-10.
Guo L, Zhang BB, Ma RJ, Cai ZX, Qian W. Effects of temperature on the pollen dissemination and germination of peach. Plant Physiol J. 2014;50:269-74.
Sutinen ML, Palta JP, Reich PB. Seasonal differences in freezing stress resistance of needles of Pinus nigra and Pinus resinosa: evaluation of the electrolyte leakage method. Tree Physiol. 1992;11:241-54.
Chang S, Puryear J, Cairney J. A simple and efficient method for isolating RNA from pine trees. Plant Mol Bio Rep. 1993;11(2):113-6.
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2−CT method. Methods. 2001;25:402-8.
Moussa HR, El-Gamal SM. Effect of salicylic acid pretreatment on cadmium toxicity in wheat. Biol Plantarum. 2010;54(2):315-20.
Antonić D, Milošević S, Cingel A, Lojić M, Trifunović-Momčilov M, Petrić M, Subotić A, Simonović A. Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought. S Afr J Bot. 2016;105:226-33.
Palta JP, Levitt J, Stadelmann EJ. Freezing injury in onion bulb cells. II. Post-thawing injury or recovery. Plant Physiol. 1977;60:398-401.
Flint HL, Boyse BR, Beattie DJ. Index of injury-a useful expression of freezing injury to plant tissues as determined by the electrolytic method. Can J Plant Sci. 1967;47:229-30.
Murray MB, Cape JN, Fowler D. Quantification of frost damage in plant tissues by rates of electrolyte leakage. New Phytol. 1989;113:307-11.
Tian F, Gong J, Zhang J, Zhang M, Wang G, Li A, Wang W. Enhanced stability of thylakoid membrane proteins and antioxidant competence contribute to drought stress resistance in the tasg1 wheat stay-green mutant. J Exp Bot. 2013;64(6):1509-20.
Kim HS, Oh JM, Luan S, Carlson JE, Ahn SJ. Cold stress causes rapid but differential changes in properties of plasma membrane H+-ATPase of camelina and rapeseed. J Plant Physiol. 2013;170(9):828-37.
Zheng YL, Li WQ, Sun WB. Effects of acclimation and pretreatment with abscisic acid or salicylic acid on tolerance of Trigonobalanus doichangensis to extreme temperatures. Biol Plantarum. 2015;59(2):382-8.
Horváth E, Szalai G, Janda T. Induction of abiotic stress tolerance by salicylic acid signaling. J Plant Growth Regul. 2007;26:290-300.
Tang M, Lu S, Jing Y, Zhou X, Sun J, Shen S. Isolation and identification of a cold-inducible gene encoding a putative DRE-binding transcription factor from Festuca arundinacea. Plant Physiol Biochem. 2005;43:233-9.
Badawi M, Daniluk J, Boucho BMH, Sarhan F. The CBF gene family in hexaploid wheat and its relationship to the phylogenetic complexity of cereals CBFs. Mol Genet Genomics. 2007;277:533-54.
Champ KI, Febres VJ, Moore BD. The role of CBF transcriptional activators in two Citrus species (Poncirus and Citrus) with contrasting levels of freezing tolerance. Physiol Plantarum. 2007;129:529-41.
Huang BO, Jin LG, Liu JY. Molecular cloning and functional characterization of a DREB1/CBF-like gene (GhDREB1L) from cotton. Sci China, Ser C, Life Sci. 2007;50:7-14.
Wisniewski M, Norelli J, Bassett C, Artlip T, Macarisin D. Ectopic expression of a novel peach (Prunus persica) CBF transcription factor in apple (Malus× domestica) results in short-day induced dormancy and increased cold hardiness. Planta. 2011;233(5):971-83.
Karimi M, Ebadi A, Mousavi SA, Salami SA, Zarei A. Comparison of CBF1, CBF2, CBF3 and CBF4 expression in some grapevine cultivars and species under cold stress. Sci Hortic. 2015;197:521-6.
Kitashiba H, Ishizaka T, Isuzugawa K, Nishimura K, Suzuki T. Expression of a sweet cherry DREB1/CBF ortholog in Arabidopsis confers salt and freezing tolerance. J Plant Physiol. 2004;161:1171-6.
Kidokoro S, Watanabe K, Ohori T, Moriwaki T, Maruyama K, Mizoi J, Yamaguchi-Shinozaki K. Soybean DREB1/CBF-type transcription factors function in heat and drought as well as cold stress-responsive gene expression. Plant J. 2015;81:505-18.