Influence of trout farm effluents on selected oxidative stress biomarkers in larvae of Ecdyonurus venosus (Ephemeroptera, Heptageniidae)

Anđelina Radojević, Dejan Mirčić, Miroslav Živić, Vesna Perić-Mataruga, Milenka Božanić, Katarina Stojanović, Jovana Lukičić, Ivana Živić

Abstract


Paper description:

  • Molecular biomarkers of oxidative stress are the most apropriate parameters for detecting early influence of pollutants. The effects of trout farms on aquatic insects at the molecular level have been scantily investigated.
  • We examined the influence of trout farm effluents on the molecular biomarkers of oxidative stress in larvae of Ecdyonurus venosus as a very sensitive bioindicator of water quality.
  • Changes in components of antioxidative defense in E. venosus proved to be reliable and sensitive biochemical biomarkers of environmental stress caused by trout farm effluents, making this species an excellent bioindicator of freshwater pollution.

Abstract: The aim of the present study was to establish the extent to which the outlet waters of trout farms affect the ecosystems of the Crnica and Skrapež rivers in Serbia. We monitored selected biomarkers of oxidative stress: superoxide dismutase (SOD), glutathione peroxidase (GPx) and total glutathione (GSH) in larvae of the species Ecdyonurus venosus, and simultaneously analyzed the changes in the physical and chemical parameters. The investigations were carried out in spring at four localities along the Skrapež and Crnica rivers: one upstream (the control localities), and three downstream from the fish farm outlets. On the Skrapež River, the fish farm was clearly visible and was markedly changed by the chemical parameters of the water, manifested as a decreased concentration of dissolved oxygen, increased concentrations of nitrates, nitrites, ammonium ions, total phosphorus and total organic carbon, and increased SOD and GPx activities and decreased GSH concentration in larvae from the first downstream locality as compared to the control locality. On the Crnica River, due to the high values of water flow (around 3 m3/s), effluents from the fish farm had no effect on the chemical parameters of the water or on the tested biomarkers.

https://doi.org/10.2298/ABS181220004R

Received: December 20, 2018; Revised: January 29, 2019; Accepted: January 30, 2019; Published online: February 1, 2019

How to cite this article: Radojević A, Mirčić D, Živić M, Perić-Mataruga V, Božanić M, Stojanović K, Lukičić J, Živić I. Influence of trout farm effluents on selected oxidative stress biomarkers in larvae of Ecdyonurus venosus (Ephemeroptera, Heptageniidae). Arch Biol Sci. 2019;71(2):225-33.


Keywords


antioxidant enzymes; biomarkers; Ecdyonurus venosus; organic pollution; oxidative stress

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