The response of weedy sunflower (Helianthus annuus L.) to nicosulfuron: an examination of vegetative parameters and acetolactate synthase activity


  • Dragana Božić University of Belgrade, Faculty of Agriculture, Nemanjina 6, Belgrade
  • Marija Sarić-Krsmanović Institute of Pesticides and Environmental Protection, Banatska bb, Belgrade
  • Ana Matković Institute of Medicinal Plant Research “Dr Josif Pančić", Belgrade
  • Filip Vranješ University of Belgrade, Faculty of Agriculture, Nemanjina 6, Belgrade
  • Snežana Jarić Institute for Biological Research ”Siniša Stanković” University of Belgrade, Bul. Despota Stefana 142, 11060 Belgrade
  • Sava Vrbničanin University of Belgrade, Faculty of Agriculture, Nemanjina 6, Belgrade


acetolactate synthase activity, dose-response, nicosulfuron, vegetative parameters, weedy sunflower


Paper description:

  • Intensive use of nicosulfuron in maize fields can lead to development of herbicide resistance, which can result in variable responses of weedy sunflower populations.
  • Effective doses of nicosulfuron and its effect on weedy sunflower acetolactate synthase (ALS) activity has not been reported.
  • This research investigated effective doses of nicosulfuron on whole weedy sunflower plant and ALS enzyme activity.
  • Weedy sunflower populations displayed very different responses to nicosulfuron, with the possibility of herbicide resistance development. This is the first examination of nicosulfuron effect on weedy sunflower ALS activity.

Abstract: Genetic and morphological interpopulation variability of weed species is often responsible for variable responses to herbicides. As weedy sunflower, an invasive form of Helianthus annuus L., possesses high morphological and genetic variability, very different responses of its populations to herbicides can be expected. This species is one of the dominant weed species in row crops, including maize, in many European countries in which nicosulfuron is intensively used for weed control. There are little available data about the response of this sunflower form to nicosulfuron or of the interpopulation variability of its response to other herbicides. The responses of three weedy sunflower populations to nicosulfuron were studied in field dose-response experiments, and acetolactate synthase (ALS) enzyme activity at different herbicide concentrations was determined in vitro. Interpopulation variability in the response to nicosulfuron was confirmed. Populations WS2 and WS3 were more that 20-fold and 30-fold less susceptible to nicosulfuron, respectively, than population WS1, based on fresh weight, whereas the differences were not so prominent based on other parameters, including plant height, leaf area and ALS activity, and ranged from 2 to 12-fold.

Received: November 6, 2018; Revised: January 18, 2019; Accepted: February 14, 2019; Published online: March 5, 2019

How to cite this article: Božić D, Sarić-Krsmanović M, Matković A, Vranješ F, Jarić S, Vrbničanin S. The response of weedy sunflower (Helianthus annuus L.) to nicosulfuron: An examination of vegetative parameters and acetolactate synthase activity. Arch Biol Sci. 2019;71(2):305-13.


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How to Cite

Božić D, Sarić-Krsmanović M, Matković A, Vranješ F, Jarić S, Vrbničanin S. The response of weedy sunflower (Helianthus annuus L.) to nicosulfuron: an examination of vegetative parameters and acetolactate synthase activity. Arch Biol Sci [Internet]. 2019Jun.4 [cited 2022Jan.19];71(2):305-13. Available from:




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