Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts

Authors

  • Ivona Veličković University of Belgrade - Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac“, Studentski trg 16, 11000 Belgrade http://orcid.org/0000-0002-7301-5583
  • Željko Žižak Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade http://orcid.org/0000-0002-0835-9275
  • Nemanja Rajčević University of Belgrade - Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac“, Studentski trg 16, 11000 Belgrade http://orcid.org/0000-0003-2260-1205
  • Marija Ivanov Department for Plant Physiology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-2480-5490
  • Marina Soković Department for Plant Physiology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-7381-756X
  • Petar D. Marin University of Belgrade - Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac“, Studentski trg 16, 11000 Belgrade http://orcid.org/0000-0002-9460-1012
  • Slavica Grujić University of Belgrade - Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac“, Studentski trg 16, 11000 Belgrade http://orcid.org/0000-0002-8287-921X

Keywords:

P. spinosa (blackthorn), antioxidant activity, antimicrobial activity, antidiabetic activity, α-glucosidase inhibitory activity

Abstract

Paper description:

  • P. spinosa (blackthorn) aqueous, ethanol and acetone fruit extracts were analyzed. Total phenolic and flavonoid contents, the anthocyanin profile and bioactivities were determined by in vitro colorimetric methods.
  • P. spinosa fruit extracts exhibited antioxidant, antimicrobial and anti-diabetic properties with the ethanol fruit extract markedly inhibiting α-glucosidase, an enzyme-linked with diabetes mellitus type II.
  • P. spinosa fruit extracts, in particular the ethanol extract, should be considered for further investigation of naturally occurring compounds with a potential anti-diabetic effect.


Abstract: We investigated the total phenolic and flavonoid contents and the anthocyanin profiles in aqueous, ethanol and acetone extracts of Prunus spinosa (Rosaceae) fruit, and their antioxidant, antibacterial, antifungal, antidiabetic and antitumor properties. The contribution of polyphenol contents to the bioactivity of the extracts was calculated and observed through Pearson’s coefficient of correlation. The acetone extract was the richest in phenols and anthocyanins and the ethanol extract in flavonoids. Cyanidin was the most abundant anthocyanin compound in all examined extracts. The ethanol extract showed the most promising antioxidant activity in DPPH, ABTS and FRAP assays. Tested bacteria were more affected by the ethanol than by the aqueous extract. Both the ethanol and aqueous extracts exhibited potential antidiabetic effects, observed as inhibition of α-amylase and α-glucosidase, enzymes linked with diabetes mellitus type II. The ethanol extract was a potent α-glucosidase-inhibitor with a significantly lower IC50 value than the positive control, glucobay, used to treat diabetes mellitus type II. Neither the ethanol nor the aqueous extracts had any effects on tested human malignant cell lines. Our results indicate that the ethanol extract showed the most pronounced in vitro antioxidant and antimicrobial effects, and a potential antidiabetic activity, which can be ascribed to its high flavonoid content. Our results indicate that research of compounds, particularly of flavonoids present in the ethanol extract and their anti-diabetic properties should be examined further.

https://doi.org/10.2298/ABS191217004V

Received: December 17, 2019; Revised: February 7, 2020; Accepted: February 8, 2020; Published online: February 10, 2020

How to cite this article: Veličković I, Žižak Ž, Rajčević N, Ivanov M, Soković M, Marin P, Grujić S. Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts. Arch Biol Sci. 2020;72(1):105-15.

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Published

2020-03-24

How to Cite

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Veličković I, Žižak Željko, Rajčević N, Ivanov M, Soković M, Marin PD, Grujić S. Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts. Arch Biol Sci [Internet]. 2020Mar.24 [cited 2022Jan.19];72(1):105-1. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4932

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